|
论文摘要目录： |
| 关键词：医药学论文 学术论文 |
【关键词】 烧伤；胰岛素；炎症；细胞因子类
Regulating effects of different doses of insulin on serum IL1β and IL10 in early stage in severely scalded rats
【Abstract】 AIM: To determine the possible effects of different doses of insulin on the proinflammatory cytokine IL1β and the antiinflammatory cytokine IL10 in early stage of severe burn in rats. METHODS: Male SpragueDawly rats (30% TBSA, full thickness burn) were randomly divided into 2 groups to receive either normal saline only (n=14, with intraperitoneal injection of 40 mL/kg normal saline, group A) or normal saline plus different doses of insulin (n=42, with intraperitoneal injection of 40 mL/kg normal saline plus subcutaneous injection of 1, 3 or 5 IU/kg insulin respectively, group B1, B2, B3). In addition, 7 rats without thermal injury were subjected to normal control group (group N). The outcome measures encompassed the serum glucose, blood pressure, serum IL1β and IL10 in rats at 6 and 24 h after scald and different treatments. RESULTS: The serum glucose increased quickly in rats after scald, which was demonstrated in group A to a range of (10.7±1.8) mmol/L by 6 h postscald. Insulin decreased the serum glucose in a dosedependent manner in rats of group B1, B2 and B3. And insulin at a dose of 3 IU/kg body weight reduced the serum glucose to a range of (6.0±0.8) mmol/L in rats at 6 h after thermal injury, which was almost eqeal to the normal range of (5.1±0.6) mmol/L (P>0.05). Meanwhile, insulin at this dose improved the blood无忧论文 【http://www.uklunwen.com】 pressure of scalded rats by keeping the blood pressure at a stable condition similar to the normal. Insulin significantly decreased the serum IL1β in a dosedependent manner in scalded rats. It was partially showed after administration of insulin of 3 IU/kg serum IL1β was (54.0±8.6) ng/L and (46.8±6.8) ng/L, much lower than that without insulin administration in rats at 6 and 24 h after thermal injury (P<0.01). Insulin could increase the serum IL10 in scalded rats. In comparison with group A, the most significant increase of serum IL10 was seen by application of 3 IU/kg insulin as (45.5±5.2) ng/L in rats 24 h after scald (P<0.01 vs group A). CONCLUSION: Our data provided an insight that insulin might attenuate the inflammatory cascade response by decreasing the proinflammatory cytokine IL1β and increasing the antiinflammatory cytokine IL10, thereby keeping or restoring the systemic homeostasis after severe burn. The best administration dose of insulin in early burn stage should be determined by normalizing serum glucose.
【Keywords】 burns; insulin; inflammation; cytokines
【摘要】 目的： 研究不同剂量胰岛素对严重烫伤早期血清IL1β及IL10的影响. 方法： 将雄性SD大鼠63只随机分为烫伤后盐水治疗（A）组、盐水 + 不同剂量胰岛素治疗（B1~B3）组，每组14只，另取7只正常大鼠作为对照（N）组. 烫伤各组在背部造成约30%体表总面积（TBSA）全层皮肤烫伤. 伤后6，24 h后检测血糖、血压及血清IL1β及IL10含量. 结果： 伤后大鼠血糖明显升高，6 h达到（10.7±1.8）mmol/L，并持续高血糖状态，胰岛素可以降低伤后血糖，并随剂量的增大而作用增强，3 IU/kg胰岛素的6 h血糖为(6.0±0.8）mmol/L，与正常值（5.1±0.6）mmol/L比较无统计学差异（P>0.05）；伤后6，24 h血压均明显低于正常值，3 IU/kg胰岛素对伤后血压有显著改善作用. 烫伤后血清IL1β显著升高，不同剂量胰岛素干预均可降低IL1β水 |
|
|
第1页 第2页 第3页 第4页 |
|
|
|
| 上一篇：不同色彩背景对选择反应和运算能力的影下一篇：Orcal下的中国大陆地热、重力网络
|
| 最新论文 |
最热门论文 |
|
|
|
|
|
